|
KMID : 0380220070400040501
|
|
Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 4 p.501 ~ p.510
|
|
|
Expressed Sequence Tag Analysis for Identification and Characterization of Sex-Related Genes in the Giant Tiger Shrimp Penaeus monodon
|
|
Preechaphol Rachanimuk
Leelatanawit Rungnapa
Sittikankeaw Kanchana
Klinbunga Sirawut
Khamnamtong Bavornlak
Puanglarp Narongsak
Menasveta Piamsak
|
|
|
Abstract
|
|
|
|
Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <10-4) whereas 308 ESTs (29.3%) were regarded as newly unidentified transcripts (E-value >10-4). A total of 559 transcripts (87 contigs and 472 singletons) were obtained. Thrombospondin (TSP) and peritrophin (79 and 87 clones accounting for 7.5 and 8.3% of clones sequenced, respectively) predominated among characterized transcripts. Several full length transcripts (e.g. cyclophilin, profillin and thioredoxin peroxidase) were also isolated. A gene homologue encoding chromobox protein (PMCBX, ORF of 567 nucleotides encoding a protein of 188 amino acids) which is recognized as a new member of the HP1 family was identified. Expression patterns of 14 of 25 sex-related gene homologues in ovaries and testes of P. monodon broodstock were examined by RT-PCR. Female sterile and ovarian lipoprotein receptor homologues were only expressed in ovaries whereas the remaining transcripts except disulfide isomerase related P5 precursor and adenine nucleotide translocator 2 were higher expressed in ovaries than testes of P. monodon broodstock. A homologue of ubiquitin specific proteinase 9, X chromosome (Usp9X) revealed a preferential expression level in ovaries than testes of broodstock-sized P. monodon (N = 13 and 11, P <0.05) but was only expressed in ovaries of 4-month-old shrimp (N = 5 for each sex).
|
|
|
KEYWORD
|
|
|
EST, Penaeus monodon, RT-PCR, Sex-specific markers, Shrimp
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|